Hydrogen Peroxide Inhibition and Inactivation of Bicupin Oxalate Oxidase
نویسندگان
چکیده
................................................................................................................ iii TABLE OF CONTENTS .............................................................................................. iv LIST OF ABBREVIATIONS ........................................................................................ x CHAPTER 1: INTRODUCTION .................................................................................. 1 Chapter 1.1: Oxalate Oxidase .................................................................................... 1 Chapter 1.2: Oxalate oxidase as a component in biofuel cells .................................. 5 Chapter 1.3: Oxalate oxidase as a potential cancer treatment ................................... 7 Chapter 1.4: Membrane inlet mass spectrometry ...................................................... 8 Chapter 1.5: Circular dichroism ............................................................................... 12 Chapter 1.6: Enzyme kinetics .................................................................................. 14 Chapter 1.7: Enzyme Inhibition Kinetics ................................................................. 16 Chapter 1.8: The motivation and novelty of this work ............................................ 21 CHAPTER 2: EXPERIMENTAL................................................................................ 23 Chapter 2.1: Materials .............................................................................................. 23 Chapter 2.2: Expression/purification of CsOxOx .................................................... 23 Chapter 2.3: Chromatographic separation of small carboxylic acid molecules by HPLC ....................................................................................................................... 24 Chapter 2.4: Observation of global structural protein changes by circular dichroism .................................................................................................................................. 25 Chapter 2.5: UV-Vis horseradish peroxidase coupled CsOxOx kinetic assay ........ 25
منابع مشابه
Hydrogen peroxide inhibition of bicupin oxalate oxidase
Oxalate oxidase is a manganese containing enzyme that catalyzes the oxidation of oxalate to carbon dioxide in a reaction that is coupled with the reduction of oxygen to hydrogen peroxide. Oxalate oxidase from Ceriporiopsis subvermispora (CsOxOx) is the first fungal and bicupin enzyme identified that catalyzes this reaction. Potential applications of oxalate oxidase for use in pancreatic cancer ...
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